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MAGE-A1、MAGE-C2及SSX-1在原发性肝细胞癌中的表达及意义
The expression of cancer-testis antigen MAGE-A1、MAGE-C2 and SSX-1 mRNA in hepatocellular carcinoma
【摘要】 目的检测肿瘤—睾丸抗原(CT抗原)MAGE-A1、MAGE-C2和SSX-1 mRNA在原发性肝细胞癌(HCC)中的表达情况。方法采用RT-PCR方法检测MAGE-A1、MAGE-C2和SSX-1 mRNA在55例HCC患者癌及癌旁组织中的表达,对产物进行测序,并对检测结果与临床指标进行分析。结果55例HCC患者的癌组织中,MAGE-A1、MAGE-C2和SSX-1 mRNA的表达率分别为60.0%、14.5%和40.0%;癌旁组织无表达。结论CT抗原MAGE-A1、MAGE-C2和SSX-1 mRNA在HCC中呈高表达,是HCC免疫治疗的理想靶位。
【Abstract】 Objective To investigate the expression of MAGE-A1,MAGE-C2 and SSX-1 mRNA in hepatocellular carcinoma.Methods Reverse-transcription polymerase chain reaction(RT-PCR) was used to investigate the mRNA expression of the three cancer-testis antigens(MAGE-A1,MAGE-C2 and SSX-1) in patients with HCC.PCR products of cancer-testis antigens were sequenced.The correlation between the expression of cancer-testis antigen and clinical parameters was analyzed.Results In 55 patients with HCC,the rate of MAGE-A1,MAGE-C2 and SSX-1 mRNA expression was 60.0%,14.5% and 40.0%,respectively.However,mRNAs of these CT antigen were not detected in the adjacent non-cancerous tissues.Conclusion MAGE-A1,MAGE-C2 and SSX-1 mRNAs are expressed with high percentages in HCC,indicating that they may be candidates for antigen-specific immunotherapy targets in HCC.
【Key words】 cancer-testis antigen; hepatocellular carcinoma; reverse-transcription polymerase chain reaction;
- 【文献出处】 山东医药 ,Shandong Medical Journal , 编辑部邮箱 ,2008年45期
- 【分类号】R735.7
- 【被引频次】3
- 【下载频次】65