【摘要】 目的研究MXA蛋白抑制HBV复制的活性。方法将pcDNA3.1-MXA重组质粒和PU19-1.24 HBV重组质粒分别按1∶1、2∶1共转染HepG2细胞(MXA组),对照组使用空pcDNA3.1、Salon DNA和PU19-1.24 HBV重组质粒共转染,3 d后Western blot检测MXA蛋白表达,Abbott法检测细胞上清HBeAg和HBsAg分泌量,定量PCR检测上清和胞内HBV DNA水平,统计学分析结果。pcDNA3.1-MXA与PU19-1.24HBV重组质粒共转染HepG2细胞,对照组为pcDNA3.1-MXA重组质粒、PU19空质粒和Salon DNA共转染,3 d后裂解细胞Western blot检测MXA蛋白表达。结果Western blot显示MXA组有MXA蛋白表达;与对照组相比,pcDNA3.1-MXA和PU19-1.24 HBV重组质粒按1∶1转染时,MXA组HBeAg下降27%,上清HBV DNA和细胞内HBV DNA分别下降1个log值和0.6个log值;按2∶1比例转染时MXA组HBeAg较对照组下降66%,上清HBV DNA和细胞内HBV DNA水平分别下降1.9个和1.7个log值,差异均具有统计学意义(P<0.05)。Western blot检测显示MXA蛋白抑制HBV组与对照组MXA蛋白表达没有明显差别。结论MXA蛋白在HepG2细胞具有抑制HBV复制活性,抑制活性与蛋白的表达量相关;在抑制HBV复制过程中MXA蛋白自身可能不发生降解。更多还原

【Abstract】 Objective To investigate the antiviral activity of interferon-induced MXA protein against hepatitis B virus.Methods The HepG2 cells in MXA groups were cotransfected with pcDNA3.1-MXA and PU19-1.24HBV in a ratio of 1 ∶ 1 or 1 ∶ 2.The control groups were cotransfected with pcDNA3.1,or salmon DNA and PU19-1.24HBV.After 3 days,the HBsAg and HBeAg in the supernatant were measured by detection kit(Abbott).The extracellular and intracellular HBV DNA was detected by real-time PCR.MXA expression was detected by Western blot.Results MXA protein was expressed in MXA groups.When the cotransfection ratio is 1 ∶ 1,compared with the control groups,HBeAg was decreased by 27%,and the extracellular and intracellular HBV DNA were decreased by 1 and 0.6 logs,respectively.When the ratio is 2 ∶ 1,compared with the control groups,HBeAg was decreased by 66%,and the extracellular and intracellular HBVDNA were decreased by 1.9 and 1.7 logs,respectively.The MXA expression shows no marked difference between group cotransfected with the MXA vector and PU19-1.24HBV and the control group.Conclusion MXA protein could inhibit the replication of HBV in the HepG2 cells and it maybe not degrade while inhibiting the HBV production.更多还原