【摘要】 为了提高玉米直链淀粉含量,用基因枪将前期工作中构建的sbeⅡb RNAi表达载体pBAC413导入玉米（Zea mays）自交系幼胚愈伤组织,经过筛选获得了12株转化再生植株,其中6株获得了结实种子。DNA点杂交、PCR扩增和PCR-Southern均证明目的基因已整合到T₀代再生植株玉米基因组中。对T₁代转基因玉米籽粒淀粉含量进行测定,结果表明总淀粉含量比对照没有显著变化,其中1个转基因玉米株系的直链淀粉含量比对照提高了15.6%。更多还原

【Abstract】 To increase the amylose content of maize(Zea mays),a RNAi vector pBAC413 with the sheⅡb of sense and antisense was constructed and transformed to maize calli induced from immature embryos of the elite inbred lines by Biolistic PDS1000/He. And twelve transformed plantlets were obtained and 6 of them were well pollinated to produce seeds.The integration of alien gene was conformed in 6 transformed plantlets by the analysis of DNA dot blot,PCR and PCR-Southem hybridization.The seed amylose content of regeneration plants was detected,and the amylose content of a transgenic line increased up to 15.6% compared with wild type.更多还原