【摘要】 根据已知的不同抗病基因保守区域设计引物,通过在水稻基因组DNA中扩增和分离有相似序列的DNA片段,可以快速鉴定出候选抗病基因。选择3对扩增条带多样性较高的引物XLRRfor/XLRRrev、S1/AS3和Ptokin1/Ptokin2,对江苏27个水稻品种进行抗病基因同源序列PCR分析(resistance gene analog-PCR,RGA-PCR)。结果显示,3对引物共扩增出清晰可辨的谱带127条,其中多态性带101条,占总数的79.53%。根据聚类分析,3对引物对或引物组合扩增的RGA图谱可将品种分为多种类型,这些RGA图谱类型与稻瘟病菌7个生理小种接种鉴定的抗病表型没有完全的对应关系。更多还原

【Abstract】 To design primers according to conserved domains in those published resistant genes,DNA sequences can be amplified from DNA genome in rice to obtain candidate resistant gene rapidly.3 primer pairs including XLRRfor/XLRRrev,Ptokin1/Ptokin2 and S1/AS3 were selected to amplify 27 rice varieties from Jiangsu Province.127 bands were amplified from 27 rice varieties by 3 primer pairs,in which 101 bands were polymorphic,with the polymorphic band frequency of 79.53%.Resistance gene analog-PCR(RGA-PCR)maps of rice varieties amplified by 3 primer pairs or primer pair combinations could be grouped by cluster analysis.There were no completely corresponding relations between these RGA-PCR map groups and resistance phenotypes of rice cultivars.更多还原