【摘要】 通过测定菌丝生长速度和子实体中镉含量,研究重金属Cd胁迫对姬松茸菌丝和子实体生长发育的影响。结果表明,菌丝生长速度随镉浓度的增加而明显受到抑制,EC50为20.2375mg·kg-1。姬松茸子实体镉的富集随培养料中外源镉浓度的增加而明显递增,符合MICHAELISMENTEN曲线回归模型;当培养料中镉添加量低于10mg·kg-1,子实体内镉的富集系数相同;当外源镉浓度超过10mg·kg-1,富集系数降低,培养料外源镉浓度至80mg·kg-1,其富集系数降至12;镉浓度超过10mg·kg-1,子实体数量显著减少;镉浓度在40mg·kg-1以上,菌丝体发育为畸形菇。因此初步认为,姬松茸子实体生长发育耐镉的临界值为40mg·kg-1。不同浓度镉胁迫其菌盖镉的富集量均比菌柄高出3倍左右。更多还原

【Abstract】 Through measuring the growth rate of mycelium and cadmium (Cd) content in fruit body, the effects of Cd stress on the mycelium and fruit body of Agaricus Blazei Murrill were studied. The results showed that the growth rate of mycelium was retarded significantly with the increase of Cd concentration in PDA with EC50 20.237 5 mg·kg-1. The accumulation of Cd in fruit body increased obviously with the increase of Cd in medium material, and the relationship between them coincided with MICHAELIS MENTEN curvilinear regression equation Y=1 387.163 1X/(X+31.579 2) (F=438.338 0**, P=0.000 1). The accumulation coefficient of Cd in fruit body was invariable when the content of Cd added in medium material was less than 10 mg·kg-1, while, which was decreased when Cd added was more than 10 mg·kg-1 and decreased to 12 when Cd added in medium material amounted to 80 mg·kg-1. Number of fruit body was significantly decreased as the content of Cd added in medium material was more than 10 mg·kg-1, and the mycelium developed and formed abnormal fruit body as Cd added was about 40 mg·kg-1. Thus, it was suggested that 40 mg·kg-1 was the critical concentration of Cd for development of fruit body. The accumulation of Cd in cap was three times more than that in stipe under different contents of Cd stress.更多还原