【摘要】 目的观察顺铂对耳廓反射正常的健康豚鼠耳蜗功能的损害作用,以探讨顺铂对豚鼠耳蜗毒性作用及其损伤机制,为防治顺铂耳毒性作用提供理论依据。方法将60只耳廓反射灵敏的健康豚鼠随机分成两组,顺铂组腹腔注射顺铂4mg/(kg.d),1次/d,连续注射5d。正常对照组腹腔注射同等剂量的生理盐水。实验前和实验5d后分别行耳廓反射和听性脑干反应测听。测定麻醉状态下豚鼠听觉脑干诱发电位,比较实验前后两组听觉诱发电位阈值及各波潜伏期、波幅变化。结果顺铂组豚鼠听性脑干反应测试听阈明显提高,各波潜伏期延长,波幅降低,甚至出现波形变异不易辨认,两组比较差异有显著性。结论顺铂可导致豚鼠耳蜗功能损害,表现为豚鼠耳蜗听性脑干反应阈值显著提高,各波潜伏期延长,波幅降低。更多还原

【Abstract】 Objective To observe the impairment of the cochlea function after using cisplatin,and to provide theoretical basis for cisplatin ototoxicity.Methods Sixty guinea pigs were randomly divided into two groups,the normal control group and the experimental group.Cisplatin with 4mg·kg-1·d-1 qd,were injected to abdominal cavity in experimental guinea pigs.The same dosage of normal saline(NS) was injected to abdominal cavity in control group animals.Before and after administration five days the auditory brainstem response(ABR) was used to measure the hearing thresholds,wave latencies,wave amplitude.Results The hearing threshold of ABR examination in the experimental group was significantly higher than that in normal group,and ABR wave latencies were longer,the difference between two groups was significant.Conclusion The cochlear hair cells and the spiral ganglion cells were impaired definitely by using cisplatin.This suggests that cisplatin has serious toxicity to the cochlear.更多还原