【摘要】 目的探讨严重急性呼吸综合征(SARS)患者肾内免疫细胞的免疫组织化学变化并对其作用进行分析。方法应用免疫组织化学方法显示6例SARS死亡患者和3例意外死亡者肾内免疫细胞,进行其形态观察,并采用图像分析系统进行定量分析。结果6例SARS患者肾内CD68+单核/巨噬细胞显著增多(P<0.05),CD3+T细胞、CD20+B细胞和S-100+树突状细胞与对照组无差别(P>0.05)。结论SARS患者肾内单核/巨噬细胞为主要免疫应答细胞,提示单核/巨噬细胞在SARS肾病变中起着重要作用。而T细胞、B细胞以及树突状细胞可能受到SARS冠状病毒的攻击,其功能可能遭到破坏。更多还原

【Abstract】 Objective To investigate the expression of the immune cells and their roles in the kidney of patients with severe acute respiratory syndrome(SARS)by immunohistochemical staining. Methods Immune cells of the kidney in 6 SARS cases and 3 control cases were labeled by immunohistochemical staining,and positive cells were morphologically observed and quantitatively analyzed by image analysis system. Results CD68 positive monocytes/macrophages increased significantly in SARS cases(P<0.05),CD3 positive T lymphocytes,CD20 positive B lymphocytes and S-100 positive dendritic cells,however,had no differences between SARS cases and control cases(P>0.05).Conclusion Monocytes/macrophages are major immune response cells in the SARS kidney which indicates that monocytes/macrophages may play an important role in the pathological changes of the SARS kidney.It is possible that T lymphocytes,B lymphocytes and dendritic cells are attacked by SARS coronavirus,and their functions may be destroyed.更多还原