【摘要】 为研究芹菜素(Apigenin,AP)对RAW264.7细胞增殖、分泌一氧化氮(nitrogen monoxidum,NO)和吞噬功能的影响,首先培养RAW264.7细胞至细胞对数生长期,然后MTT法检测不同浓度(25、50、100、150和200μmol/L)的AP对脂多糖(Lipopolysaccharide,LPS)刺激的RAW264.7细胞增殖的影响,再用Griess试剂盒检测上述浓度AP对LPS刺激的RAW264.7细胞分泌NO的影响,并用荧光微球检测其吞噬功能的变化.结果显示25~200μmol/L AP显著抑制LPS刺激的RAW264.7细胞的增殖,并明显抑制LPS刺激的RAW264.7细胞分泌NO,同时明显抑制LPS刺激的RAW264.7细胞的吞噬功能(P<0.01),且呈剂量依赖关系.故在一定浓度范围内,AP显著抑制LPS刺激的RAW264.7细胞增殖、分泌NO和吞噬功能,故有望通过进一步研究将其开发成为免疫调节药物.更多还原

【Abstract】 To study the effect of Apigenin(AP) on the proliferation and nitric oxide(NO) secretion and phagocytosis of RAW264.7 cells,RAW264.7 cells were cultured at the log phase,MTT method tested the effect of different concentrations(25,50,100,150 and 200 μmol/L) of AP on proliferation of cells in response to LPS;Griess kit measured the effect of those concentrations of AP on NO secretion of RAW264.7 cells in response to LPS,and the change of phagocytosis was detected by fluorescent microbeads.Those results showed that 25~200 μmol/L of AP inhibited the proliferation of RAW264.7 cells in response to LPS,and inhibited NO secretion of RAW264.7 cells in response to LPS,and inhibited the phagocytosis of RAW264.7 cells obviously(P<0.01) in a dose-dependent manner.So AP can inhibit the proliferation and NO secretion and phagocytosis of RAW264.7 cells in response to LPS obviously,promising to be developed into an immunoregulation drug by further studies.更多还原