【摘要】 用紫外光谱法、荧光光谱法研究异鼠李素与牛血清白蛋白的相互作用机理及几种金属离子与异鼠李素和牛血清白蛋白之间的相互作用,并探讨了几种金属离子与异鼠李素结合的类型,阐明了它们的结合产物对牛血清白蛋白的作用机理.实验结果表明,异鼠李素与牛血清白蛋白主要是凭借静电引力作用结合,异鼠李素主要以静态猝灭方式使牛血清白蛋白荧光强度减弱.金属离子的介入影响了异鼠李素与牛血清白蛋白的结合,降低了其结合能力.更多还原

【Abstract】 The interaction mechanisms of Isorhamnetin(ISO) and bovine serum albumin(BSA),ISO and several metal ions as well as BSA and several metal ions were studied by means of ultraviolet-visible absorption spectrometry and fluorescence spectroscopy.These experimental results show that the static gravitation was the major force of the combination about the interaction of ISO and bovine BSA.Flourescence of BSA was quenched by ISO with the static mechanism.The binding styles of several metal ions and ISO were studied,too.It shows the binding mechanisms of some complexes of the metal ions and ISO with BSA.It indicates that the interventions of metal ions affected the combination of ISO and BSA,they depressed their abilities of combination.更多还原