【摘要】 目的探讨IFN-α联合不同浓度As2O3对NB4细胞株血管内皮生长因子(VEGF)、凋亡抑制蛋白(Sur-vivin)表达的影响。方法实验分不同浓度的As2O3即0、0.1、0.5、1.0、2.0μmol/L及不同浓度As2O3联合IFN-α共10组,利用MTT法检测细胞毒性反应,同时采用免疫组织化学方法半定量分析NB4细胞VEGF、Survivin表达的动态变化。结果Survivin在未加药组阳性率为93.8%,VEGF在未加药组阳性率为82.4%。As2O3(≤1.0μmol/L)作用后VEGF表达增加,但无统计学意义;Survivin表达下降(P<0.029)。2.0μmol/L As2O3作用下两者表达均明显降低(P=0.006;P=0.000);联合IFN-α组两者表达均明显降低(P=0.000),分析两者呈正相关(r=0.8754)。结论IFN-α联合As2O3可降低VEGF、Survivin的表达,提示IFN可以降低As2O3对NB4细胞凋亡抑制基因的表达,具有调控细胞凋亡的作用。更多还原

【Abstract】 Objective To investigate the expressions of vascular endothelial growth factor(VEGF) and Survivin in NB4 cells lines affected by interferon-α combining with arsenic trioxide.Methods NB4 cells line were treated with arsenic trioxide alone(with doses of 0,0.1,0.5,1.0,2.0μmol/L)or in combination with interferon-α.Cytotoxic reaction was assessed by MTT assay.The dynamic expressions of VEGF and Survivin were detected by semi-quantitative immunohistochemistry.Results The positive rates of VEGF and Survivin in control group were 82.4% and 93.8%,respectively.VEGF expression increased under effect of arsenic trioxide(≤1.0μmol/L)(no statistically significance),instead,Survivin decreased(P<0.029).VEGF and Survivin decreased when treating with 2.0μmol/Larsenic trioxide,as well as combining with interferon-α.Positive correlation was observed between of them(r=0.8754).Conclusion Interferon-α combining with arsenic trioxide can down regulate the expressions of VEGF and Survivin and suggests that interferon-α can decrease the expression of inhibitor of apoptosis gene in NB4 cell line and play a role in the regulation of cell apoptosis.更多还原