【摘要】 目的采用HRP-HPLC测定中药红曲中的微量毒性成分桔霉素。方法用复合提取剂提取红曲样品,采用Irrogulour-C18柱(250 mm×4.6 mm,10μm);流动相为乙腈-水(15:85,pH2.8);流速1 ml.min-1;柱温25℃;荧光检测,波长λex=331 nm、λem=500 nm。结果桔霉素7~220 ng与峰面积的线性关系良好(r=0.9999),最低检测限0.5 ng,相对回收率99.8%~103.2%,日内和日间RSD分别为1.76%~3.31%、3.96%~5.02%。16批红曲发酵物中的桔霉素平均含量为21.4±19.1μg.kg-1。结论所用方法灵敏准确、简便、重复性好、专属性强,可用于中药红曲中桔霉素的检测。更多还原

【Abstract】 OBJECTIVE To establish RP-HPLC method for the content determination of citrinin concentration in Monascus.METHODSMonascus was extracted with the mixtrue of extractants.Citrinin was separated by the Irrogulour-C18 column using methanol-water(15:85,pH2.8) as mobile phase with the flow rate 1 ml·min-1,the detection wavelength besetat λex=331 nm,λem=500 nm,and column temperature at 25℃.RESULTS The linear range of the method was 7-220 ng(r=0.9999).The lowest detectable limit was 0.5 ng and the average recovery was 99.8%-103.2% with with-in day and between-day RSD of 1.76%-3.31% and 3.96%-5.02%,respectivelly.average citrinin content of 16 batches of Monascus was 21.4±19.1 μg·kg-1.CONCLUSION The established method with the advantages of simplicity,precision,high sensitivity and high repeatability,and is suitable for the detection of citrinin concentration in Monascus.更多还原