【摘要】 目的研究5’-磷酸腺苷(5’-AMP)体外抗氧化和对体外氧化损伤脾细胞的损伤修复能力。方法用化学比色法测定5’-AMP体外清除二苯代苦味酰基自由基(DPPH自由基)的能力;建立过氧化氢(H2O2)氧化损伤体外培养小鼠脾细胞模型,用MTT法检测5’-AMP修复受损伤脾细胞的作用,并分析其对细胞抗氧化体系及抗氧化能力的影响。结果5’-AMP具有剂量依赖性的体外抗氧化和清除活性氧能力,添加0·5mmol/L、1mmol/L、5mmol/L和10mmol/L5’-AMP均能显著修复H2O2诱导的脾细胞氧化损伤(P<0·05),总抗氧化能力和抗氧化酶类活力(P<0·01),5’-AMP添加量大于1mmol/L时,可显著降低丙二醛(MDA)含量(P<0·01)。其细胞培养液的氧自由基(ROS)水平逐渐降低,5’-AMP添加量为10mmol/L时,ROS水平接近对照组水平。结论5’-磷酸腺苷能显著修复氧化损伤,具有显著的抗氧化作用。更多还原

【Abstract】 Objective To investigate in vitro antioxidation of 5’-adenylic acid(5’-AMP) and its repairing effect on oxidative damage in spleen cells.Methods Scavenging of DPPH free radicals by 5’-AMP was studied by chemical colorimetry.Mouse splenic cells were treated with H2O2 to induce oxidative stress injury,which was determined.The effect of 5’-AMP on injury was studied by MTT method.Total antioxidation capability(T-AOC),catalase(CAT) activities,glutathione peroxidase(GSH-Px) activities,diaminodiphenyl methane(MDA) and reactive oxygen species(ROS) level in the incubation medium were detected.Results 5’-AMP could scavenge DPPH radicals in a concentration dependent manner in vitro and significantly repair the injury by H2O2 in splenic cells.Compared to that of the untreated group,the number of living cells increased significantly in H2O2-injured splenic cells with the supplementation of 0.5,1,5 and 10mmol/L 5’-AMP(P<0.05).CAT activities,GSH-Px activities and T-AOC in the incubation medium were increased significantly with 5’-AMP concentration increase(P<0.01),while MDA content decreased significantly(P<0.01) with the supplementation of more than 1mmol/L 5’-AMP.ROS level decreased gradually,and 10mmol/L of 5’-AMP obtained the best effect,which was close to the control group.Conclusion 5’-AMP has a significant anti-oxidation activity,which can significantly repair the oxidative injury.更多还原