【摘要】 叶绿体的发育与功能以及叶片的光合作用代谢会受到植物生长调节剂(PGRs,plant growth regulators)的重要调节。光诱导延迟荧光(DF,delayed fluorescence)被认为是叶绿体光系统Ⅱ(PSII)可逆光化学反应的结果,是PSⅡ光化学效率的灵敏探针。本文以蚕豆为实验材料,利用自行研制的便携式延迟荧光生物传感器,研究了八种不同作用模式的PGRs对蚕豆幼苗DF特征的影响。结果发现,绿色植物叶片光诱导DF强度的变化可以准确的反应PGRs对蚕豆植株生长和光合作用代谢的调控效应。因此,DF技术有望成为农业生产中PGRs对作物生长发育调控效应早期、实时、在位监测的一个便捷、无损的新方法。更多还原

【Abstract】 With the lamina of broad bean(Vicia faba) as testing models,a portable delayed fluorescence(PF) biosensor is designed to study the effects of eight plant growth regulators(PGRs) with different modes of action on the characteristics of DF.The results demonstrate that the changes in DF intensity of green plant can truly reflect the regulatory effects of PGRs on the growth and photosynthetic metabolism of broad beam seedlings.Therefore,the DF biosensor technology is anticipated to be a simple and non-invasive method for early and real-time monitor the regulatory effects of PGRs on plant growth and development in vivo.更多还原