【摘要】 研究了草地早熟禾愈伤组织的诱导和分化的最适培养基,建立了早熟禾高频率的再生体系。结果表明:品种优异的胚性愈伤诱导率最高,其中含2.0 mg/L 2,4-D和0.2 mg/L6-BA的培养基处理胚性愈伤组织的诱导频率最大,可以达到65.26%。早熟禾品种肯塔基、午夜、优异与新哥莱德最适分化激素浓度与配比分别为6-BA 1.0+KT 0.26、-BA 2.0+2,4-D 0.1、6-BA1.0+2,4-D 0.2、KT 2.0,愈伤组织分化率分别达到27.93%、52.38%、66.67%和60%。早熟禾再生芽在1/2 MS+0.2 NAA培养基上生根率可达到100%。更多还原

【Abstract】 The best culture medium of the callus induction,differentiation were studied.The plant regeneration system has been established.The results showed that embryo callus inducing ratio of Merit was the highest,reached 65.25% on the modified MS medium containing 2.0 mg/L 2,4-D and 0.2 mg/L 6-BA.The best callus differentiation medium of Kentuky、Midningt、Merit and Nuglade was different due to different genotype,The best callus differentiation medium of Kentuky、Midningt、Merit and Nuglade was 6-BA 1.0+KT 0.2,6-BA 2.0+2,4-D 0.1,6-BA 1.0+2,4-D 0.2,KT 2.0 individually,the ratio of callus differentiation of the Kentuky、Midningt、Merit and Nuglade was 27.93%、52.38%、66.67% and 60%.The rate of rooting of Poa pratensis L.on half strength MS medium+0.2 NAA reached 100%.更多还原