【摘要】 目的:了解医院获得性金黄色葡萄球菌(HA-SA)和社区获得性金黄色葡萄球菌(CA-SA)所携带肠毒素、表皮剥脱性毒素(ETs)、中毒性休克综合征毒素-1(TSST-1)及杀白细胞素基因(PVL)的特点。方法:应用PCR法检测肠毒素A、B、C、D、E基因,ETs A、B基因,TSST-1以及PVL。结果:116株金黄色葡萄球菌中,85株为HA-SA,检出肠毒素A基因6株,肠毒素C基因1株,肠毒素D基因1株,PVL2株;31株为社区获得性金黄色葡萄球菌,检出肠毒素A基因1株,肠毒素B基因1株,肠毒素D基因1株,PVL7株;所有菌株均未检测到肠毒素E、ETs A、B和TSST-1基因。CA-SA PVL检出率显著高于HA-SA,两者比较差异有统计学意义(P<0.005),而肠毒素、ETs和TSST-1基因检出率比较差异均无统计学意义(P>0.05)。结论:金黄色葡萄球菌可分泌多种毒素,在分离金黄色葡萄球菌的同时,应注意其毒素检测。更多还原

【Abstract】 Objective:To study genic feature of Staphylococcal enterotoxins(SEs),exfoliative toxins(ETs),toxic shock syndrome toxin 1(TSST-1) and Panton-Valentine leukociclin(PVL) carried by both hospital-acquired Staphylococcus aureus(HA-SA) and community-acquired Staphylococcus aureus(CA-SA).Methods:The genes of SEA-SEE,ETA,ETB,TSST-1 and PVL were detected by PCR.Results:Out of 116 SA strains,85 strains were HA-SA.SEA was detected in 6 strains,SEC in 1 strain,SED in 1 strain,and PVL in 2 strains.Thirty one strains were CA-SA.SEA was detected in 1 strain,SEB in 1 strain,SED in 1 strain,and PVL in 7 strains.SEE,ETA,ETB and TSST-1 were negative in all SA.PVL positive rate detected in CA-SA was much higher than that in HA-SA(P<0.005),while positive rates of SEs,ETs,TSST-1 were not significant different between CA-SA and HA-SA(P>0.05).Conclusions:Staphylococcus aureus may secrete various toxins,detecting should pay attention to whose toxins while separating Staphylococcus aureus.更多还原