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植物激素与芦荟原汁对丽格海棠组培快繁的影响
Effects of Plant Hormones and Aloe Juice on Rapid Propagation in Rieger begonia
【摘要】 [目的]研究植物激素和芦荟原汁对丽格海棠丛生芽诱导的影响。[方法]以丽格海棠叶片为外植体,研究了不同浓度的6-BA和NAA和芦荟原汁对丽格海棠丛生芽诱导的影响。[结果]不同浓度的NAA对丽格海棠丛生芽的诱导有明显影响,NAA浓度为0.5 mg/L的诱导效果最好(83.3%),随着NAA浓度的下降,诱导率也随之下降;6-BA不同浓度对丛生芽诱导有一定影响,但影响程度不大,以浓度为0.5 mg/L的诱导效果较好(66.7%);在一定的植物激素浓度下,添加适宜浓度的芦荟原汁对丛生芽的诱导有明显的促进作用,以MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+3%芦荟原汁和MS+6-BA 0.5 mg/L+NAA 0.5 mg/L+1%芦荟原汁的诱导丛生芽的效果较好,诱导率均为100%。[结论]6-BA和NAA和芦荟原汁以适宜的浓度搭配可缩短丽格海棠的出芽时间,提高丛生芽的诱导率。
【Abstract】 [Objective] The aim was to study the effects of the plant hormones and aloe juice on the clustered buds of Rieger begonia.[Method] With the leaves of R.Begonia as tested materials,the effects of plant hormones(NAA and 6-BA) with different concn.and aloe juice on the induction of the clustered buds in R.Begonia.[Result] NAA with different concn.had obvious effect on the induction of the clustered buds in R.Begonia.The induction of NAA at 0.5 mg/L was best(83.3%)and was decreased along with the dropping of NAA concd.6-BA with different concn.had some effect on the induction of the clustered buds in R.Begonia,but the effect was not great,and 6-BA at 0.5 mg/L got better induction effect(83.3%).Appropriate concn.of the aloe juice added in medium with certain concn.of plant hormones could distinctly promote the induction of the clustered buds of R.Begonia.The media of MS+ 6-BA 0.5 mg/L+ NAA 0.1 mg/L+ aloe juice 3% and MS+ 6-BA 0.5 mg/L+ NAA 0.5 mg/L+ aloe juice 1% got better induction effect,with induction of 100%.[Conclusion] The combination of 6-BA,NAA and aloe juice with appropriate concn.could shorten the induction time and increase the induction rate of the clustered buds in R.Begonia.
【Key words】 Rieger begonia; Phytohormone; Aloe juice; Tissue culture; Rapid propagation;
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2008年20期
- 【分类号】S682.29
- 【被引频次】1
- 【下载频次】173