【摘要】 [目的]探讨木质素高效降解菌血红密孔菌产酶的最佳条件。[方法]利用平板显色法比较5株白腐菌降解木质素的能力,得到了一株优势菌株——血红密孔菌,研究其在培养过程中的生长,产酶情况及其木质素降解酶的最佳培养条件。[结果]该菌株能够产生两类木质素降解酶,且酶的热稳定性较好。不同温度,碳氮源,pH值,表面活性剂对产酶的影响较大,通过正交试验得出该菌株在液体培养基中的最佳培养条件为培养温度38℃,初始pH=4.5,葡萄糖浓度10 g/L酒石酸铵浓度1.0 g/L.[结论]该研究可为生产实践中利用该菌降解木质素提供一定的理论依据。更多还原

【Abstract】 [Objective] The purpose was to study the optimum conditions of Pycnoporus sanguineus for producing ligninase.[Method] A strain of lignindegrading white-rot fungus was selected from 5 strains of collected fungi and its ligninase production and the optimum conditions for producing ligninolytic enzyme were measured.[Result]It could produce two kinds of ligninase with good thermal stability.Different temperatures,carbon sources,nitrogen sources,acidities,as well as the additions of surfactant had distinct influence on the development of lignin-degrading enzymes of the fungus.The optimum condition was drawn out: 38 ℃,pH=4.5,10 g/L glucose,1.0 g/L tartaric acid ammonium.[Conclusion] The aim of the research was to provide a basis for lignin degradation in practice.更多还原