【摘要】 [目的]寻求提高大戟组培苗成活率的途径。[方法]研究了3种内生真菌、不同浓度的琼脂和活性炭对药用植物大戟组培苗生长和炼苗成活率的影响。[结果]接种大戟的内生真菌E5(Fusariiumsp.)能显著促进大戟组培苗的生长,使其炼苗后的成活率达100%。大戟株高和平均生根数在琼脂为14g/L、活性炭为0.05g/L的培养基中最高,分别为(4.56±0.67)cm和(3.9±0.6)条,炼苗成活率达68%。当培养基琼脂为8g/L和活性炭为0时,大戟组培苗的生物量、主根根长和叶片数最高,分别为(0.267±0.053)g、(6.35±0.68)cm和(13.8±1.7)片,成活率达70%。与培养基成分的变化相比,添加内生真菌对组培苗的影响更显著。[结论]植物自身的内生真菌有利于进一步提高组培苗的成活率,这对于大戟大量繁殖具有积极的意义。更多还原

【Abstract】 [Objective] The study aimed to seek for approachs of increasing the survival rate of tissue culture seedlings in Euphorbia pekinensis. [Method] The effects of 3 kinds of endophytic fungi, the agar and activated carbon with different concentration on the growth of tissue culture seedling of E. pekinensis and their survival rates of transplanting and exercising were studied. [Result] The endophytic fungi E5 inoculated on E. pekinensis could promote growth of tissue culture seedlings of E. pekinensis, with the survival rate of exercising seedlings being up to 100 %. When the medium was with agar and active carbon of 14 and 0.05 g/L, resp., the plant high and mean roots of E. pekinensis seedlings were highest, being(4.56±0.67) cm and (3.9±0.6) resp., and the survival rate of exercising seedlings reached 68 %. When the medium was with 8 g/L agar and without active carbon, the biomass, main root length and leaf number in tissue culture seedling of E. pekinensis was(0.267±0.053) g, (6.35±0.68) cm and(13.8±1.7), with the survival rate of plantlet being 70 %. Compared with the change of the mediums component, the effect adding endophytic fungi on growth of tissue culture seedling was more obvious.[Conclusion] The endophytic fungi in plant itself can favor to enhance the survival rates of tissue culture seedling further, which has active meaning for mass reproduction of E. pekinensis. and it has great value for widely culturing of Euphorbia pekinensis.更多还原