【摘要】 [目的]研究甲醇和抗坏血酸对蚕豆生理特性的影响。[方法]用1.5%甲醇、10mmol/L抗坏血酸(ASA)和自来水(CK)喷洒蚕豆幼苗,测定叶片的呼吸速率、相对电导率、超氧阴离子含量和多酚氧化酶(PPO)的活性。[结果]各处理的相对电导率和呼吸速率均增大。甲醇、ASA处理蚕豆叶片的相对电导率、超氧阴离子含量和PPO的活性均低于CK。甲醇处理的相对电导率最小,ASA处理的超氧阴离子含量和PPO活性最小。超氧阴离子的含量与测量时的温度及呼吸速率有关。甲醇处理花开前期出现了呼吸高峰,花开期呼吸速率有所下降但变化平缓,ASA处理蚕豆叶片的呼吸速率始终低于CK。[结论]甲醇和ASA处理蚕豆叶片的相对电导率较小,保持了细胞膜的完整性,抑制了PPO的活性,延缓了蚕豆衰老。更多还原

【Abstract】 [Objective] The purpose was to study the effects of methanol and ascorbic (ASA) on the physiological characteristics of Vicia faba. [Method] The V. faba seedlings were sprayed with 1.5 % methanol, 10 mmol/L ASA and tap water (CK), and the respiration rate, relative conductance, superoxide anion content and polyphenoloxidase (PPO) activity in leaves were determined. [Result] Both relative conductance and respiration rate increased in each treatment. The relative conductance, superoxide anion content and PPO activity in V. faba leaves treated with methanol and ASA were lower than that in CK. The relative conductance was smallest in methanol treatment and the superoxide anion content and PPO activity were least in ASA treatment. The superoxide anion content related to temperature and respiration rate while measuring. The respiration peak appeared in the period before flowering in methanol treatment and the respiration rate decreased a little with slow change in flowering period. The respiration rate in V. faba leaves treated with ASA was always lower than that in CK. [Conclusion] The relative conductance in V. faba leaves treated with methanol and ASA was less, which kept the integrity of cell membrane, restrained PPO activity and delayed V. faba consenescence.更多还原