【摘要】 目的分析乙型肝炎(乙肝)患者及其配偶、子女血清中的HBV-DNA和血清标志物的检测结果。方法ELISA法和荧光PCR法进行HBVM(乙肝血清标志物5项)与HBV-DNA的测定。结果HBV-DNA的阳性率和含量在HBcAg(+)与HBeAg(-)组差异有统计学意义(χ2=18.21,P<0.01;χ2=156.11,P<0.01)。乙肝患者配偶的HBV感染率为50.56%,母婴与父子女之间的传播差异有统计学意义(χ2=357.97,P<0.01)。结论HBeAg(+)与HBV-DNA的含量有密切关系,母婴传播远高于父子女传播,家庭传播是导致乙肝、肝硬化、肝坏死和肝癌的主要原因之一,而配偶之间的密切接触可感染HBV,但很少引起乙肝。更多还原

【Abstract】 [Objective] To analyze the detection result of the HBV serum markers among the hepatitis B patients and their family members.[Methods]ELISA and fluorescence PCR were adopted to determine HBVM and HBV-DNA.[Results]Significant difference was found in the HBV-DNA positive rate and content between the HBeAg(+) group and HBeAg(-) group(P<0.01);the HBV positive rate among the patients’ spouses was 50.56%;the difference was significant between mother-infant spread and father-infant spread(P<0.01).[Conclusion]HBeAg(+) is closely related with the content of HBV-DNA;mother-infant spread is much more serious than father-infant spread;spread among family members is one of the major causes of hepatitis B,hepatic necrosis,cirrhosis and hepatic cancer;close contact between spouses might lead to HBV infection but seldom causes hepatitis B.更多还原