【摘要】 目的:通过研究掌叶大黄中主要成分大黄酸、芦荟大黄素、大黄素、大黄素甲醚和大黄酚清除超氧阴离子自由基(O2.-)的作用,从分子生物学的角度探讨大黄延缓衰老作用的可能机理。方法:采用电子自旋共振技术(ESR)分别检测大黄中主要成分对O2.-的清除作用。结果:大黄中主要成分对O2.-的清除作用,依次为大黄酸>大黄酚>大黄素>大黄素甲醚>芦荟大黄素。结论:大黄所具有的延缓衰老的作用,可能与其所含的主要成分大黄酸、芦荟大黄素、大黄素、大黄素甲醚和大黄酚具有清除O2.-的能力有关。更多还原

【Abstract】 Objective:To study the scavenging effects of Rhein,Aloe-emodin,Emodin,Physcion and Chrysophanol which were the main ingredients in Rheum palmatum L.on superoxide anion radical and to investigate the mechanism of Rheum palmatum L.in retarding aging at molecular biology lever.Methods: The electron spin resonance(ESR)was used to observe the scavenging effect on the superoxide anion radical(O2.-)of the main ingredients in Rheum palmatum L.Results: All of the Rhein,Aloe-emodin,Emodin,Physcion and Chrysophanol were effective in scavenging O2.-produced in the alkaline DMSO system,and the ability was Rhein>Chrysophanol> Emodin>Physcion>Aloe-emodin.Conclusion:Rheum palmatum L.has the effect of retarding aging.Perhaps this is relevant to the scavenging effects on the superoxide anion radical(O2.-)of the main ingredients in the herb.更多还原