【摘要】 目的:观察当归多糖硫酸酯(APS)的抗氧化损伤作用。方法:常规方法体外培养Hela细胞,加入不同浓度的APS孵育24h后,用H2O2或紫外线照射诱导细胞氧化损伤。APS抗氧化损伤作用检测如下:MTT法测定细胞活力;比色法测定胞浆内SOD活力(黄嘌呤氧化酶法)、GSH和MDA。结果:H2O2或紫外线照射损伤的Hela细胞活性明显下降,胞浆中的GSH、SOD活性显著降低,MDA含量升高,与对照组比有显著性差异(P<0.05或P<0.01),表现出明显的氧化损伤特点。APS在0.3~100μg/m1浓度范围内,剂量依赖性地增加细胞活力,升高GSH、SOD的活性,降低MDA的含量,与模型组比有显著性差异(P<0.05或P<0.01)。结论:APS具有抗细胞氧化损伤的作用,这可能是其抗AIDS作用的机理之一。更多还原

【Abstract】 Objective: To investigate the anti-oxidative effect of Angelica polysaccharide sulphate（APS）.Methods: The Hela cells were cultured conventionally. Then APS was added and cultured together in difierent concentration for 24h followed by a oxidative injury with H₂O₂ or UV irradiation.The anti-oxidative effects of APS were detected as follow:cell viability was measured by MTT assay;colorimetric analysis was used to determine SOD activity,GSH and MDA level in cytoplasm.Results: Treatment of H₂O₂ or UV irradiation significantly decreased cell viability,GSH and SOD activity in cytoplasm,while increased MDA in cytoplasm.At the range of 0.3～100μg/ml,APS significantly increased cell viabilty,GSH and SOD activity,while decreased MDA in a dose dependent manner（P<0.05 or P<0.01）.Conclusion: APS has anti-oxidative effect,which may be one of its anti-AIDS mechanisms.更多还原