【摘要】 目的:利用高效毛细管电泳(HPCE)技术建立人工蛹虫草药材的指纹图谱。方法:采用50 cm×75μm毛细管柱,以0.5 mmol/L硼砂溶液为缓冲液,运行电压为20 kv,检测波长为254 nm,柱温25℃。结果:建立的指纹图谱中共有11个共有峰,且方法学考察符合规定的标准。结论:该法准确简便,可作为控制人工蛹虫草中药材内在质量的有效手段。更多还原

【Abstract】 Objective: To investigate the High Performance Capillary Electrophoresis(HPCE) fingerprint of cultured Cordyceps militaris(L.) Link.Methods: Separation was performed on a 50 cm×75 μm uncoated capillary with 0.5 mmol/L borate solution(pH 9.18) as HPEC buffer.The run voltage was 20 Kv,temperature 25℃ and the DAD detection was set at 254nm.Results: Fingerprint consisted of 11 common peaks.The validation of methods was satisfied with the requirements for SFDA’s technical regulations.Conclusion: The method was accurate and simple and suitable to the quality control of cultured Cordyceps militaris(L.) Link.更多还原