【摘要】 目的观察端粒酶基因(hTR、hTRT)、凋亡相关基因(p53、bcl-2)在乳腺癌及癌前病变中的表达及相互关系。方法应用原位杂交方法检测端粒酶基因(hTR、hTRT)和凋亡相关基因(p53、bcl-2)mRNA在44例乳腺导管非典型增生组织中的表达,应用免疫组化方法检测p53基因蛋白在上述病例中的表达,并与6例乳腺良性乳腺增生及26例乳腺癌病例进行了比较。结果端粒酶基因(hTR、hTRTmRNA)在乳腺导管重度非典型增生组织中表达增强(60.9%,52.1%),其表达与在轻、中度非典型增生中(分别为22.2%,11.1%和33.3%,25.0%)和乳腺癌组织中(88.5%,80.8%)的表达差异显著(P<0.05).p53mRNA在乳腺导管非典型增生组织中的表达随着异型性的增加而下降,而p53基因蛋白则相应增加。Bcl-2在乳腺导管非典型增生组织中呈中度表达,其中以在重度中表达明显。结论端粒酶基因hTR、hTRT的表达与乳腺非典型增生细胞的恶性转化密切相关,同时可检测到p53mRNA的表达缺失、p53蛋白突变及bcl-2mRNA的过表达,且表达水平与端粒酶活性有相关关系。更多还原

【Abstract】 [Objective] To investigate the expression and the relationship between telomerase genes(hTR,hTRT) and apoptosis related genes(p53, bcl-2) in breast ductal cancer and precancerous lession. [Methods] Expressions of telomerase genes (hTR,hTRT), p53, and bcl-2 genes mRNA were detected by in situ hybridization, and the expression of the p53 protein was detected by immunohistochemistry. 44 patients with ductal atypical hyperplasia were selected and compared with 6 of benign hyperplasia and 26 of breast ductal cancer. [Results] The expression of telomerase genes (hTR, hTRT mRNA) in severe ductal atypical hyperplasia was enhanced (60.9%,52.1%), and significantly different from those in mild-medium ductal atypical hyperplasia (22.2%, 11.1%; 33.3%, 25.0%)and breast ductal cancer(88.5%, 80.8%) (P <0.05). With the upgrading of atypia of atypia of ductal hyperplasia, expression of wild p53 mRNA (mild: 55.6%; medium: 41.7%; severe: 26.1%) decreased while expression of the p53 protein (mild: 11.1%: medium: 25.0%; severe: 34.8%) increased. As for bcl-2 mRNA, it showed moderate expression more obviously in severe atypical hyperplasia. [Conclusion] There is a significant correlation between the expression of telomerase genes(hTR, hTRT) and the state of malignant transformation in ductal atypical hyperplasia. The absence of p53 mRNA, the mutation of p53 protein and the overexpression of bcl-2 mRNA, which associate with telomerase activation, can be detected.更多还原