【摘要】 目的观察糖尿病(diabetes mellitus,DM)大鼠海马神经元β-淀粉样蛋白(amyloid betapeptide,Aβ)的变化。方法用链脲佐菌素(streptozotocin,STZ)建立DM大鼠模型,用32P放射性配体结合实验检测各组糖原合酶激酶-3(glycogen synthase kinase-3,GSK-3)的活性。用免疫组织化学的方法分别检测对照组、DM组、DM+Li2CO3组Aβ的表达。结果与对照组相比,DM组GSK-3的活性明显升高(P<0.01),Aβ表达明显增加(P<0.05),碳酸锂处理后GSK-3的活性明显降低(P<0.01),Aβ的表达下调(P<0.05)。结论DM大鼠海马GSK-3的活性异常增加,可能诱导Aβ的表达增加。更多还原

【Abstract】 Objective To investigate the alteration of Aβ peptide in diabetic rat hippocampus.Methods The diabetic rats were induced by streptozotocin.The activity of glycogen synthase kinase-3 was measured by ³²P-labelling and the expression of Aβ was determined by immunohistochemistry in control group,DM group,DM+ Li₂CO₃ group.Results The activity of GSK-3 was stimulated（P<0.01）,the expression of Aβ was high（P<0.05） in DM group. After the DM rats were treated with Li₂CO₃,the activation of GSK-3 was decreased and the Aβ expression was reduced.Conclusion In DM rat brain,the activation of GSK-3 leads to overexpression of Aβ,and Li₂CO₃ might reduce the expression of Aβ by inhibiting GSK-3.更多还原