【摘要】 采用RT-PCR技术从野生种马铃薯中克隆到一个光敏色素基因PHYB,其cDNA全长为3470bp。含有一个3393 bp的完整开放阅读框,编码一条长1130个氨基酸的蛋白,分子量为125kDa,等电点为5.6。该基因编码的蛋白序列与栽培种马铃薯、番茄和烟草同源基因编码的氨基酸序列一致性分别为98%、95%、92%,命名为SpPHYB.半定量PCR分析表明,根、茎、叶和芽中SpPHYB表达水平较高且相似,但在花和块茎成熟器官中表达量稍低.更多还原

【Abstract】 The full-length cDNA of SpPHYB gene was cloned from wild potato (Solanum pinnatisectum) by RT-PCR.The cDNA was 3470 bp long with an open reading frame (ORF) of 3 393 bp and encoded 1 130 amino acid residues with a predicted molecular weight of 125 kDa and an isoelectric point of 5.6.The protein se- quence Comparison showed that the predicted protein had higher identities to those from Solanum tuberosum, Lycopersicon esculentum and Nicotiana tabacum (98%,95% and 92%).Semi-quantitive RT-PCR indicated that the expression level of SpPHYB was higher and similar in roots,stem,leaves and sprouts,and lower in flowers and tubers.更多还原