【摘要】 目的:研究海(水)产品中霍乱弧菌新的增菌分离检验方法,提高方法的最小检出量及结果的准确性和可靠性。方法:海(水)产品等水生动物检样经搅碎后,应用四硼酸钠缓冲溶液,将污染在组织中的霍乱弧菌等微生物洗脱溶释游离在液体内,吸取上层清液加入5倍浓碱性蛋白胨,霍乱弧菌在特定的条件下进行有效增菌,然后分离鉴定。结果:四硼酸钠能有效地控制增菌条件。pH值变异系数0.63%,减少干扰物质组胺75.0%~80.0%,最小检出量0.01~0.04 cfu/g,比原方法可提高5个对数的阳性检出量,检出率提高50%以上。结论:本方法具有防腐、抗干扰强、增菌条件稳定、分离平板上杂菌少、菌落易识别、菌型不易变异、操作方便和阳性检出率高的特点,可缩短检测周期1倍,节约成本65.0%~70.0%。不但适用于海(水)产品,也适用于其它固体食品的检测。更多还原

【Abstract】 Objective:To improve the sensitivity,accuracy and reliability of testing methods of Vibrio cholera in marine products.Methods:The samples of marine product were smashed and washed with borax buffer.The upper solution was drawn,and basic peptone was put in.The germ was amplified,separated and identified.Results:Borax was effective to control germ amplification.The coefficient of pH variation was 0.63%,histamine was reduced by 75.0%~80.0%,the sensitivity was 0.01~0.04 cfu/g,the rate that Vibro cholera was properly test increased by more than 50%.Conclusion:This method is characterized by antiseptic,antiinterference,steadiness,convenience and high rate that Vibrio cholera was properly test.The testing period is reduced by half,the cost is reduced by 65.0%~70.0%,and this method is also suitable for testing other solid food.更多还原