【摘要】 目的:对12株疑似O157:H7大肠菌采用PCR法进行鉴定。方法:利用单一PCR和多重聚合酶链反应(mPCR)检测不同来源菌株志贺样毒素(stx1和stx2)、溶血素(hly)、粘附抹平因子(eaeA)、β-葡糖醛酸糖苷酶(u idA)、O157抗原编码(rfbE)、H7鞭毛抗原编码(fliC)基因。结果:4株大肠菌rfbE和fliC基因检测为阳性,确认为EHEC O157:H7,其中1株菌株扩增出全部毒力基因,另3株菌株扩增出除stx1外其它全部毒力基因;2株大肠菌rfbE基因检测阳性,确认为O157:H7-大肠菌;其它均为非O157:H7其它大肠菌。结论:PCR技术的应用能对可疑O157:H7大肠菌进行有效鉴定与分析,应成为今后病原学鉴定的主要技术手段。更多还原

【Abstract】 Objective:To identify 12 strains of suspectable E.coli O157:H7 by using PCR.Methods:Seven genes of stx1,stx2,hly,eaeA,uidA,rfbE,fliC from different strains were detected by PCR and multiplex PCR.Results:Four strains were E.coli O157:H7,one of which amplified all virulence genes and the other 3 amplified all virulence genes except stx1.Two strains were E.coli O157:H7-and the rest were E.coli non-O157:H7.Conclusion:Qualitative detection of PCR can effectively identify and analyze suspectable E.coli O157:H7,which can be used as main method of etiological and epidemiological identification.更多还原