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用Vero细胞从病人痰液中分离呼吸道合胞病毒的实验研究

Laboratory study on RSV isolated from children patients′ sputum through vero cells

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【作者】 郑官增； 朱函坪； 裘丹红； 沈伟伟； 王敬；

【Author】 Zheng Guan-zeng~1,Zhu Han-ping~2,Qiu Dan-hong~1,Shen Wei-wei~1,Wang Jing~1 (1.Taizhou Center for Disease Control and Prevention,Taizhou 318000,China;2.Zhejiang Center for Disease Control and Prevention,Hangzhou 310009,China)

【机构】 浙江省台州市疾病预防控制中心； 浙江省疾病预防控制中心； 浙江省台州市疾病预防控制中心 浙江台州318000； 杭州310009； 浙江台州318000；

【摘要】 目的:证实本次暴发流行的病原和Vero细胞分离RSV的效果,获得可用于疫苗研制的RSV毒株。方法:用Ve-ro细胞从患儿痰标本中分离RSV并对分离到的毒株进行生物学特性研究。结果:从13例患儿痰标本中分离到3株可稳定传代的病毒株,经RSV单克隆抗体、RT-PCR扩增产物的核酸序列测定及CPE特征等证实为RSV。结论:本次婴幼儿呼吸道疾病暴发是由RSV A型所引起,Vero细胞分离RSV阳性率为23.1%。

【Abstract】 Objective:To confirm pathogen causing children′s respiratory tract infection and evaluate the effects that RSV is isolated through vero cells.Methods:RSV from children patients′ sputum was isolated through vero cells and the RSV biological features were also studied.Results:Three strains of virus that can proliferate stably were isolated from 13 children patients′ sputum.RSV was conformed through its CPE features,RSV monoclonal antibody and nucleotide sequencing technology.Conclusion:The children′s respiratory diseases were caused by RSV-A.RSV positive rate is 23.1% through vero cells.

【关键词】 呼吸道合胞病毒； Vero细胞；
【Key words】 Respiratory syncytial virus(RSV)； Vero cells；
  • 【文献出处】 中国卫生检验杂志 ,Chinese Journal of Health Laboratory Technology , 编辑部邮箱 ,2007年01期
  • 【分类号】R373
  • 【被引频次】2
  • 【下载频次】162
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