【摘要】 目的探讨精子凋亡与精子密度、活力、形态关系。方法分析69例男性不育患者精液样本,采用计算机辅助精液分析进行精液参数检测,采用精子形态检测系统下人工修正方法进行精子形态分析,瑞-姬染色检测精子凋亡率。结果精子凋亡率与精子密度之间没有显著相关性(P>0.05),精子凋亡率与精子活力、正常形态精子百分率之间有显著负相关性(P<0.01,P<0.05);活力异常组精子凋亡率高于活力正常组,两组比较差异有显著性(P<0.05);严格标准下,形态异常组精子凋亡率高于形态正常组,两组比较差异有显著性(P<0.01)。结论精子凋亡率与精子活力和正常形态精子有相关性,精子凋亡率增加可能是弱精子症和畸形精子症患者不育的原因之一。更多还原

【Abstract】 Objective To study the relationship between percentage of sperm apoptosis and sperm concentration,motility and morphology.Methods Sperm samples were obtained from 69 patients of male infertility.Semen parameters were detected by computer assisted sperm analysis(CASA).The sperm morphology analysis using semiautomatic sperm morphology analyzer was performed.The semen smears were stained by Wright-Giemsa staining for analyzing percentage of sperm apoptosis.Results There was a significantly negative relative between the percentage of sperm apoptosis and sperm motility and normal morphology(P<0.01,P<0.05).The percentage of sperm apoptosis of abnormal motility group was significantly higher than that of normal motility group(P<0.05).The percentage of sperm apoptosis of abnormal morphology(strict criteria) group was significantly higher than that of normal morphology group(P<0.01).Conclusion The percentage of sperm apoptosis is negatively related with sperm motility and normal morphology.Sperm apoptosis could be one of the causes of asthenozoospermia and teratozoospermia.更多还原