【摘要】 研究新型重组H5N1亚型禽流感灭活疫苗对种鸡和肉鸡的免疫原性,并对雏鸡母源抗体和免疫后的抗体进行动态观察,根据试验结果推荐该疫苗对鸡的免疫程序。用HI方法检测种鸡、肉鸡的母源抗体和免疫抗体,根据母源抗体的衰减和免疫抗体的消长规律确定首免和再免日龄。结果表明种鸡的母源抗体约能维持10 d多;0.3 mL/羽首免后10 d HI抗体就可达到6.40 log2,3-5周达到高峰期,至免疫17周后(19周龄)HI抗体水平仍然维持在4.88 log2;19周龄时0.5 mL/羽进行二免,有效抗体能维持约20周;280日龄0.5 mL/羽三免后抗体水平均一,下降缓慢,至种鸡淘汰时(三免后29周)抗体水平仍能维持在5.32 log2。肉鸡母源抗体约能维持7d,10日龄时0.3 mL/羽免疫,有效抗体能维持到上市。新型重组H5N1亚型禽流感灭活疫苗对鸡的免疫原性确实。更多还原

【Abstract】 Serum antibodies in chickens were detected by HI test,that were induced by recombinant highly pathogenic avian influenza(HPAI) inactivated vaccine(H5N1 Subtype),and the eating and flowing laws of the vaccine-induced antibodies and the vaccine dosage was also examined.The maternal antibody could last 10 days in breeding chickens.The HI antibody was 6.40log2,and the peak value appeared in week 3 to 5.The HI antibody could maintain 4.88log2 in week 19.Then the second immune injection adopted 0.5 mL/bird,the effective antibody could last 20 weeks.The third immune injection of 0.5 mL/bird on day 280,the antibody level was steady 5.32log2 till the bird being culled out.The maternal antibody could last 8 days in broiler chickens.The dose of neck hypodermic immune was 0.3 mL/bird on day 10,the antibody was effective even when chickens be carried into market.So the recombinant AI inactivated vaccine(H5N1 Subtype) was effective in preventing AIV challenge for chickens.更多还原