【摘要】 【目的】研究一氧化氮(NO)和乙烯处理对肥城桃内源乙烯生物合成的影响,并探讨NO与乙烯的拮抗作用。【方法】分别用10μl·L-1NO和1000μl·L-1外源乙烯、10μl·L-1NO和1000μl·L-1外源乙烯共同处理3种方式处理肥城桃果实,研究果实内源乙烯生物合成的变化。【结果】外源乙烯促进了肥城桃果实的内源乙烯生物合成;而NO通过显著降低果实中ACS和ACO的活性,明显抑制了肥城桃果实内源乙烯的生物合成。NO和外源乙烯共同处理的肥城桃果实内源乙烯生物合成低于外源乙烯单独处理而高于NO单独处理,表明NO对外源乙烯具有拮抗作用。【结论】NO抑制了果实内源乙烯的生物合成,且抑制了外源乙烯对果实乙烯生物合成的催化作用。更多还原

【Abstract】 【Objective】The effects of nitric oxide (NO) and exogenous ethylene on ethylene biosynthesis in harvested ‘Feicheng’ peaches were studied. The antagonistic actions between NO and exogenous ethylene was also explored.【Method】 ‘Feicheng’ peaches were fumigated with 10 μl·L-1 NO, 1 000 μl·L-1 ethylene, or 10 μl·L-1 NO plus 1 000 μl·L-1 ethylene for 3 h. 【Result】Application of exogenous ethylene promoted the biosynthesis of endogenous ethylene in peach fruit. The treatment with NO inhibited remarkably the biosynthesis of ethylene by significantly reducing the activities of ACC synthase (ACS) and ACC oxidase (ACO). Ethylene biosynthesis in the fruits treated with both NO and exogenous ethylene was lower than those with exogenous ethylene, but it was higher than those with NO, suggesting that there were antagonistic actions between NO and exogenous ethylene.【Conclusion】NO could inhibit the biosynthesis of ethylene and the catalysis of exogenous ethylene on the ethylene biosynthesis in peach fruits.更多还原