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生防菌BH-2发酵液中活性代谢产物的抑菌作用及理化性质

Study on Antifungal Action and Physicochemical Characteristics of Bioactive Metabolite Produced by Biocontrol Bacterial Strain BH-2 Fermentation

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【作者】 梁建根竺利红吴吉安桑金隆姚杭丽施跃峰

【Author】 Liang Jiangen,Zhu Lihong,Wu Ji’an,Sang Jinlong,Yao Hangli,Shi Yuefeng(Institute of Plant and Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021)

【机构】 浙江省农业科学院 植物保护与微生物研究所浙江省农业科学院植物保护与微生物研究所杭州310021

【摘要】 为了明确生防菌BH-2的抑菌作用和抑菌因子的相关性质,采用管碟法,对其发酵液中活性代谢产物的抑菌作用及理化性质进行了研究。结果表明,发酵液中活性代谢产物具有较广的抗菌谱,对多种植物病原菌均表现较好的抑制作用。此外,活性代谢产物耐121℃高温,在偏酸性或中性条件下稳定,有较强的抗紫外线照射能力,对蛋白酶K和胰蛋白酶稳定性较好,54℃下15d的稳定性较好。这为该菌株的进一步研究和产品开发奠定了坚实的基础。

【Abstract】 A study on antifungal action and physicochemical characteristics of bioactive metabolite produced by biocontrol bacterial strain BH-2 fermentation was launched by means of cylinder plate method for the sake of evaluating the antifungal action and relative properties of inhibitive factors of strain BH-2.The results showed that the bioactive metabolite had a broad antifungal spectrum and exhibited antagonistic abilities to several plant pathogens.In addition,it could endure the treatment of a high temperature of 121℃,was stable at acidic,neutral,54℃ for 15d respectively,maintained the antifungal activity after treatment of proteinase K,trypsin and ultraviolet radiation.These provided substantial foundation for a further research and production development of strain BH-2.

  • 【文献出处】 中国农学通报 ,Chinese Agricultural Science Bulletin , 编辑部邮箱 ,2007年09期
  • 【分类号】S476.1
  • 【被引频次】17
  • 【下载频次】264
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