【摘要】 mRNA差异显示技术是分析差异表达基因的强有力的工具。本实验针对油菜差别显示体系筛选优化,最终获得了差别显示体系。研究结果为:RNA模板在2μg,反转录产物用量为1.5μl时,可以扩增出较多且清晰的条带;PCR退火温度在40℃扩增条带清晰。该体系用于甘蓝型油菜低磷胁迫前后基因差异表达研究稳定可靠。更多还原

【Abstract】 mRNA differential display is a powerful instrument to indentify differentially expressed genes.This experiment is designed to optimize a differential display with silver staining of oilseed, and a optimized differential display system is obtained. The results showed :Many and clear bands were observed ,When the amount of RNA used was about 2μg and the amount of reverse transcription products used was 1.5μl. The annealing temperature on DDRT-PCR Amplification was 40℃,which produced many cDNA band. This system which was used for the analysis of gene expression under low phosphorus condition in Brassica napus L. is stable and reliable.更多还原