【摘要】 为给板栗的分子遗传育种工作提供重要的分子数据参考,作者通过cDNA-AFLP的方法,以同株板栗幼年花序和叶片为实验材料,在转录水平上进行差示分析。结果显示:64对引物组合共产生了2131条PCR产物,对其中的110条克隆测序,发现有28条基因与已知功能基因相关,其中包括参与植物转运﹑代谢﹑能量产生相关的蛋白,从而证明这些基因在板栗生长发育中的重要作用。更多还原

【Abstract】 In order to provide more references for chestnut molecular breeding, authors used the method of cDNA-AFLP to analyze gene expression profiles between inflorescences and leaves of chestnut. The results showed that: sixty-four combination of AFLP primers produced 2,131 fragments; twenty-eight genes showed significant homolog to known genes according to the sequencing of one hundred and ten genes, including transport facilitation, metabolism and energy-related proteins, which indicate the importance of them.更多还原