【摘要】 荔枝幼胚诱导的胚性培养物在低糖条件下连续继代4～6次左右,可筛选到颗粒细小、不含原胚的松散型胚性愈伤组织;以这种松散的胚性愈伤组织作为起始材料,在附加2,4-D2mg/L或2,4-D2mg/L、KT1mg/L、AgNO35mg/L的MS液体启动培养基上振荡培养(100～120r/min)10～14d,即可建立起分散性良好的胚性悬浮细胞系。采用激素减半的2种启动培养基交替继代培养或周期性固体-液体轮回培养,可以长期保持胚性悬浮细胞系。荔枝胚性悬浮细胞在附加NAA0.1mg/L、KT或Ze5mg/L、肌醇100mg/L、蔗糖50g/L、琼脂10g/L的MS固体培养基上诱导体胚,25～40d后可形成大量胚状体,诱导体胚数量达10,000个/gFW以上。经过成熟培养后,正常的体胚75%以上萌发再生完整植株。更多还原

【Abstract】 The friable embryogenic callus with small grains and without proembryo formation was screened in the process of continuous subcultures for 4～6 times on the medium with low content of sucrose from embryogenic cultures induced from immature embryos in litchi(Litchi chinensis Soon.).The well-scattered embryogenic cell suspensions could be rapidly established from the friable embryogenic callus in 2 liquid initial MS media respectively supplemented with 2,4-D 2 mg/L or 2,4-D 2 mg/L,KT1 mg/L and AgNO3 5 mg/L shaking at 100～120 r/min within 10-14 days.Long-term maintenance of the embryogenic cell suspensions was achieved via alternative cultures in the 2 revised initial medium only with half-strength of phytohormones or through the method of periodical in-turn liquid-solid culture.The embryogenic suspension cells formed somatic embryos at the frequency of over 10,000 embryoids/g FW on the solid MS medium amended with NAA 0.1 mg/L,KT or Ze 5 mg/L,sucrose 50 g/L,agar 10g/L and myo-inositol 100 mg/L within 25～40 days.Normal somatic embryos were converted into plantlets by over 75% after maturation.更多还原