【摘要】 目的探讨苦参碱对人宫颈癌细胞株Hela细胞体外增殖的抑制作用。方法选用人宫颈癌Hela细胞进行体外培养,以0.25～4.0mg/ml的苦参碱分别处理Hela细胞24～72h后,用MTT法检测细胞生长抑制率,流式细胞仪检测细胞凋亡率,倒置显微镜和透射电镜进行形态学观察。结果苦参碱对Hela细胞有抑制作用,并呈剂量和时间依赖性;流式细胞仪检测到细胞在苦参碱作用后,细胞凋亡率增加;透射电镜观察发现部分细胞发生凋亡早期改变。结论苦参碱对人宫颈癌Hela细胞具有明显抑制作用,诱导细胞凋亡可能是其机制之一。更多还原

【Abstract】 Objective To investigate the inhibitory effect of matrine on human cervical carcinoma Hela cells in vivo.Methods Cultured Hela cells were treated with different concentrations of matrine solution(0.25～4.0mg/ml)for 24～72 h.MTT assay was used to evaluate the cytotoxic effect of matrine on Hela cells.Flow cytometry was used to detect the apoptosis of Hela cells.Cell morphology was observed with transmission electron microscope.Results Different concentrations of matrine were cytotoxic to Hela cell in a time-dependent and dose-dependent manner.The FCM analysis showed that the apoptosis rate of Hela cells was increased after treatment of matrine.Hela cells presented the characteristic morphological changes of apoptosis under inverted microscope and transmission electron microscope.Conclusion Matrine can inhibit the proliferation of human cervical carcinoma Hela cells in vitro,which is associated with cell apoptosis.更多还原