【摘要】 选用16头体重60 kg左右杜长大三元杂交母猪,分为两组,分别饲喂01、00 mg·kg-1N-甲基-天冬氨酸(NMA),研究NMA对肥育猪脂肪组织甘油三酯脂肪酶活性及其mRNA表达的影响.结果表明,试验7 d后,肥育猪脂肪组织甘油三酯脂肪酶活性略有提高,但差异不显著;15 d后,酶活性显著提高,较对照组增加30.44%(P<0.05);甘油三酯脂肪酶mRNA丰度变化与其酶活性变化呈相同趋势,7 d后无显著改变,但15 d后,mRNA丰度显著提高13.70%(P<0.05).由此可知,NMA可以通过调控猪脂肪组织甘油三酯脂肪酶酶活性及mRNA的表达,从而影响猪机体脂肪代谢.更多还原

【Abstract】 Sixteen female pigs(Duroc×Landrace×Yorkshire) with about 60 kg were group-fed(eight pigs per group) the basal diets supplemented with 0(control),100 mg·kg-1 N-methyl-D-aspartate(NMA) to evaluate the efficacy of NMA on adipose tissue glyceride lipase acitivity and mRNA expression.Compared with the control,the results showed that 100 mg·kg-1 NMA could improve the swine glyceride lipase activity and mRNA expression obviously after 15 days,which were increaseed by 30.44%(P<0.05) and 13.70%(P<0.05),respectively,but did not have significant effects after 7 days.The results indicated NMA could affect the fat metabolism by manipulation of glyceride lipase acitivity and mRNA expression.更多还原