【摘要】 以水稻、褐飞虱及其卵期重要寄生蜂稻虱缨小蜂为模式系统,研究了水杨酸与过氧化氢信号转导途径在褐飞虱为害诱导的水稻挥发物释放中的作用.结果表明,褐飞虱为害能迅速地导致水稻体内水杨酸(为害后1h)和H2O2(为害后1.5h)含量的上升,早于褐飞虱为害诱导水稻释放引诱稻虱缨小蜂挥发物所需的时间.外用水杨酸和H2O2处理的试验结果表明,水稻生理浓度范围内的水杨酸处理能诱导水稻释放引诱稻虱缨小蜂的挥发物,而H2O2则只有在远高于水稻生理浓度时才能诱导.水杨酸或H2O2处理的稻株挥发物对寄生蜂的引诱作用要弱于褐飞虱为害的稻株,并且在挥发物组成相方面亦与后者存在比较明显的差异,尽管它们之间显示了一些相似的变化.这些结果说明水杨酸信号转导途径参与了褐飞虱为害诱导的水稻挥发物的释放,而H2O2信号转导途径在其中的作用则可能相对较小;同时,也表明了这些挥发物的释放除了水稻体内水杨酸信号转导途径的参与外,还可能涉及到其它的信号转导途径.更多还原

【Abstract】 Using a model system consisting of rice plants, rice brown planthopper Nilparvata lugens and its main egg parasitoid Anagrus nilaparvatae, the role of salicylic acid and H₂O₂ signaling pathways in the production of rice volatiles induced by N. lugens was studied. The result showed that N. lugens infestation quickly enhanced the levels of salicylic acid （SA） （1 h after infestation） and H₂O₂ （1 .5 h after infestation） in rice plants, which was prior to the emission of N. lugens-induced rice volatiles attractive to the parasitoid. Exogenous application of SA, whose levels were within the physiological concentrations of rice plants, on rice plants resulted in the release of volatiles attractive to the parasitoid, whereas H₂O₂ did not until its levels applied on rice plants were far higher than the physiological concentration. The volatiles emitted from SA-treated plants or H₂O₂-treated plants showed weaker attractiveness to the parasitoid compared to those from the herbivore-infested plants. Moreover, there were obvious differences in volatile profiles between the herbivore-infested plants and SA-treated plants or H₂O₂-treated plants, although they showed some similar changes after elicitation. These suggest that SA signaling is involved in the production of rice volatiles induced by N. lugens, while H₂O₂ signaling might be not. Moreover, the results also demonstrate a possible involvement of other signaling pathways in the production of the volatiles except for the SA signaling.更多还原