【摘要】 从患病鸭群中分离到一株引起鸭产蛋锐减而不死亡的病毒,经鉴定该病毒属于Ⅰ型禽副粘病毒,命名为YH99V株。以YH99V株的基因组RNA为模板,用RT-PCR一步法扩增出其F基因的cDNA序列,然后将其克隆至pMD18-T载体中,对其进行序列测定。结果表明,所克隆的基因片段长度为1 750 bp,含有1个1 662bp的开放式阅读框架(ORF),编码553个氨基酸。将YH99V株F基因序列和推导的氨基酸序列与国内外新城疫毒株的F基因相应序列比较后发现,它们的核苷酸序列同源性分别在82.7%-99.6%,氨基酸序列同源性为87.2%-99.3%。YH99V株在F蛋白裂解位点区的氨基酸序列与弱毒株在这一区域的序列完全相同,表明为弱毒株。根据F基因的全核苷酸序列,进一步绘制了Ⅰ型禽副粘病毒株F基因的系统发育树。分析结果显示YH99V株与Lasota等参考毒株同属于基因Ⅱ型,表明它们具有较近的亲缘关系。更多还原

【Abstract】 A strain virus was isolated from duck.It resulted to egg laying drop ,but didn’t cause duck to die.The virus was identified as avian paramyxovirus1 and designated as YH99V.Using the viral genome RNA as template,cDNA fragments of F gene of strain YH99V was amplified by one-step RT-PCR,and which was cloned into pMD18-T vector and sequenced.The sequence analysis showed that the nuclotide sequence of the F gene was 1 750 bp,and it contains a single open reading frame(ORF),which encodes a polypeptide of 553 amino acids.The nucleotide sequence of F gene and the deduced amino acid sequence of F protein of strain YH99V were compared with the published sequences of F gene of NDV strains,and the homology found was between 82.7%-99.6% in nucleotide sequence,87.2%-99.3% in deduced amino acid sequence.The study on cleavage site amino acid sequence deduced from the nucleotide sequence(residues 112-117) showed that YH99V strain matched to NDV lentogenic strains.Based on alignments of nucleotide sequences of F gene the phylogenetic tree was constructed.The analysis showed that YH99V strain and Lasota reference strain belong to genotypeⅡ,and indicated that they had a common origin.更多还原