【摘要】 从采自宁波北仑的马尾松中分离到一种伞滑刃属线虫,经形态学观察、测量,以及ITS-RFLP图谱比对,鉴定为松材线虫。从原木中分离到的线虫雌虫未见尾端完全呈钝圆的个体,多数都有尾尖突,长约0.5～2.9μm,平均长度约为1.7μm。经实验室灰葡萄孢培养后,雌虫尾端均呈钝圆,其形态特征与相关描述一致。这表明寄主和环境因素对线虫的形态特征有较大影响,应在一致的培养环境下进行形态观察和描述,ITS-RFLP方法是伞滑刃属线虫鉴定有效的辅助手段。更多还原

【Abstract】 One Bursaphelenchus species was isolated from Pinus massoniana at Beilun,Ningbo.It was identified as B.xylophilus by observation on its morphological characters,measurement and ITS-RFLP method.Most of the females detected from wood had clear macro of about 0.5 ~ 2.9 μm,average 1.7μm.After cultured in Botrytis cinerea,however,its morphological characters were quite the same as normal description.It proved that the host tree and environment could have influence on morphological characters.更多还原