【摘要】 目的探讨医务人员和住院患者鼻前庭携带金黄色葡萄球菌(SAU)的相互关系和对发生与控制医院感染的影响。方法对医院发生SAU感染较多科室的医务人员和住院>1周患者的鼻前庭进行采样,常规方法对SAU鉴定和耐甲氧西林金黄色葡萄球菌(MRSA)确认;K-B纸片扩散法进行药敏试验并分型;对SAU进行随机引物扩增多态DNA检测并做同源性分析;比较SAU的耐药谱分型和随机扩增多态性DNA(RAPD)基因分型之间的差异,判断医务人员和住院患者鼻前庭携带SAU的相关性。结果从290份医务人员和患者鼻前庭标本中分离出45株SAU,根据药敏试验结果,按耐药模式45株细菌分为5型;RAPD聚类分析可以将本组细菌分为8个类型。结论部分医务人员和患者之间以及患者与患者之间鼻前庭携带的SAU有较高的同源性。更多还原

【Abstract】 OBJECTIVE To investigate the relationship of epidemic strains of meticillin-resistant Staphylococcus aureus(MRSA) in vestibules of noses among medical staff and inpatients.METHODS The S.aureus strains and strains of MRSA were isolated from the vestibules of noses among medical staffs and inpatients.The genes of the isolated strains were amplified by randomly amplified polymorphic DNA(RAPD) assay.RESULTS The 45 strains of S.aureus could be divided into 5 types according to their drug-resistance,and into 8 types according to their homology.CONCLUSIONS The S.aureus strains from medical staff,inpatients,and infected patients are highly homological.更多还原