【摘要】 目的探讨5-烯丙基-7-二氟亚甲基白杨素（ADFMChR）通过活化过氧化物酶体增殖因子激活受体（PPARγ）诱导人卵巢癌细胞凋亡作用的分子机制。方法采用 MTF 法测定 ADFMChR对卵巢癌（CoCl）细胞系细胞增殖抑制作用;碘化丙啶染色流式细胞术（FCM）检测 ADFMChR 诱导CoCl 细胞凋亡程度;DNA 琼脂糖凝胶电泳证实 ADFMChR 诱导 CoCl 细胞凋亡作用。Western 印迹检测 ADFMChR 对 CoCl 细胞 PPARγ,NF-kB,Bcl-2,Bax 蛋白表达的影响。结果 MTT 法显示ADFMChR 呈剂量依赖性抑制 CoCl 细胞增殖作用,IC₅₀值为7.76 μmol/L;FCM 分析 ADFMChR 呈剂量依赖性诱导 CoCl 细胞凋亡,ADFMChR（10.0,30.0 μmol/L）处理 CoCl 细胞48 h,凋亡率分别为33.07%和73.70%,均高于相应浓度白杨素诱导凋亡率（21.70%、40.00%）;ADFMChR（30 μmol/L）处理 CoCl 细胞48 h 的 DNA 琼脂糖凝胶电泳呈现典型梯形条带,加用 PPARγ阻断剂（GW9662）后条带消失;Western 印迹分析 ADFMChR 以剂量依赖方式上调 CoCl 细胞 PPARγ和 Bax 蛋白表达,下调NF-kB 和 Bcl-2蛋白表达。结论 ADFMChR 通过活化 PPARγ抑制 NF-kB 和 Bcl-2表达,上调 Bax,诱导 CoCl 细胞凋亡。更多还原

【Abstract】 Objective To investigate induction of apoptosis of human ovarian cancer CoC1 cells by 5-Allyl-7-Gen-Difluoromethylenechrysin（ADFMChR）in vitro,and its molecular mechanism.Methods The proliferative inhibition of CoC1 ceils treated with ADFMChR was measured using（3,4,5-dimethyhhiazol-2- yl）-2,5-diphenyl tetrazolium bromide（MTT）colorimetric assay.The apoptosis of CoC1 cells induced by ADFMChR was determined by DNA agarose gel electrophoresis assay and flow cytometry using PI staining. Effect of ADFMChR on PPARγ,NF-κB,Bcl-2,Bax protein expression level of CoC1 cells was detected by Western blotting.Results The proliferation of CoC1 cells could be significantly inhibited by ADFMChR in a dose-dependent manner,The IC₅₀was 7.76 μmol/L.ADFMChR significantly induced apoptosis in a concentration-dependent,the rate of apoptosis was 33.07% and 73.70% respectively after treatment with 10.0,30.0 μmol/L of ADFMChR for 48 h,which was higher than either the control group（21.70%, 40.00%）at the same concentration ChR-treated cells.The ladder-shaped band could be shown in DNA agarose gel electrophoresis after treatment with ADFMChR at 30.0 μmol/L for 48 h and the ladder-shape band disappeared with GW9662.Western Blot analysis shown that expression of PPARγ and Bax proteins were upregulation and protein levels of NF-KB and Bcl-2 were depress after treatment with ADFMChR in a concentration-dependent.Conclusion The effect of ADFMChR on induction of apoptosis in CoC1 cells may be mediated by activation of PPARγ,sequentially accompanied by reducing of protein levels of NF-κB and Bcl-2 and increasing of Bax expression.更多还原