【摘要】 目的探讨 RCAS1与雌激素受体亚型在正常子宫内膜、增生症内膜和内膜癌组织中的表达及相关性,分析它们在内膜癌发生发展中的作用。方法采用免疫组化、RT-PCR 法检测20例正常子宫内膜组织、42例子宫内膜增生症组织及50例内膜癌组织中 RCAS1和雌激素受体α(ERα)雌激素受体β(ERβ)的表达,免疫印迹法检测各组内膜中 RCAS1蛋白的表达。结果免疫组化显示RCAS1在正常、简单型和复杂型增生内膜中均为 P 表达模式,不典型增生内膜中30%(6/20)为 D 表达模式,内膜癌中均为 D 模式。正常内膜、简单型和复杂型增生、不典型增生、内膜癌 RCAS1蛋白高水平表达率分别为0%(0/20),9.1%(2/22),45.0%(9/20),68.0%(34/50),各组间差异有统计学意义(P<0.05)。RCAS1蛋白高表达与患者肌层侵犯深度、脉管侵犯、ERα阳性相关(P<0.05)。免疫印迹法、RT-PCR 结果与免疫组化结果一致。RCAS1 mRNA 的表达水平与 ERα mRNA 呈显著正相关,而与 ERB mRNA 表达未显现相关性。结论 RCAS1高表达与表达模式的改变可能参与子宫内膜从良性到恶性转变的过程,RCAS1协同 ERα表达与子宫内膜癌的发展、浸润转移有关。更多还原

【Abstract】 Objective To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) and estrogen receptor subtypes in normal,hyperplastic,and carcinomatous endometrium and to explore their possible roles in carcinogenesis and progression of endometrial carcinoma.Methods Immunohistochemistry and semi-quantitative reverse tanscriptase-polymerase chain reaction (RT-PCR) were applied to detect the protein and mRNA expression of RCAS1,estrogen receptor (ER) α and ERβ in 20 specimens of normal endometrium,24 specimens of hyperplastic endometrium,and 50 specimens of cacinomatous endometrium.Western blotting was used to detect the RCAS1 protein expression.Results Normal endometrium and simple and complex hyperplastic endometrium showed a P pattern of RCAS1 expression;carcinomatous endometrium showed the D pattern;and the atypical hyperplastic endometrium showed both,30% (6/20) of which showed D pattern.The high level RCAS1 protein expression rates of the normal,simple and complex hyperplasia,atypical hyperplasia,and endometrial carcinoma were 0% (0/20),9.1% (2/22),45.0% (9/20),and 68.0% (34/50) respectively with a significant difference between any 2 groups (all P<0.05).The high level expression of RCAS1 was detected more frequently in the carcinomatous endometrium with deep myometrial invasion, vascular invasion,and positive of ERα (all P<0.05).The Western blotting and RT-PCR results were correlated with the immunohistochemistry results.The RCAS1 mRNA level was positively correlated with the ERαmRNA level(P<0.01),but not correlated with the ERβmRNA level(P>0.05).Conclusion The expression and distribution of RCAS1 may be involved in the malignant transformation of endometrium,and RCAS1 coexpression with ERα may be associated with the development and metastasis of endometrial carcinoma.更多还原