【摘要】 目的探讨 T 淋巴瘤侵袭转移诱导因子1(Tiam 1)在胃癌组织、癌旁组织、胃良性病变组织中的表达水平,及其与胃癌转移等生物学行为的关系。方法用实时荧光定量逆转录 PCR(FQ-RT-PCR)方法,对66例胃癌患者的癌组织、癌旁组织及11例胃良性病变患者的组织标本中Tiaml mRNA 进行定量检测。结果 66例胃癌患者的癌组织、癌旁组织中 Tiaml mRNA 表达阳性率分别为92.4%、19.7%,11例胃良性病变患者的组织标本中有1例患者有 Tiaml mRNA 表达,3类组织标本的差异有统计学意义(x~2=78.96,P<0.01);胃癌组织中 Tiaml mRNA 表达量高于癌旁组织,且与患者性别、年龄、肿瘤大小、肿瘤原发部位无关(P>0.05),但随着淋巴结转移的发生、浸润深度加深、分化程度降低、TNM 分期增高,而呈现增高趋势(P<0.05)。结论 FQ-RT-PCR 检测 Tiaml 表达具有较高的敏感性和特异性,Tiaml 与胃癌生物学行为密切相关,有可能成为胃癌转移的基因治疗新靶点。更多还原

【Abstract】 Objective To evaluate the expression quantity of T lymphoma invasion and metastasis inducing factor 1(Tiara 1)in gastric cancer tissues,tissues around the cancer over 3 cm and tissues of gastric benign lesions,and to analyze the correlation between amount of Tiaml and gastric cancer biological behaviours.Methods Real-time fluorescence quantitive RT-PCR(FQ-RT-PCR)was used to detect the expression level of Tiaml mRNA in 66 cases of gastric cancer tissues,tissues around the cancer over 3 cm and the gastric tissues of 11 cases with benign lesion.Results Positive rate of Tiam1 mRNA expression in gastric cancer tissues,tissues around the cancer was 92.4%,19.7%,respectively,and only one case express Tiaml mRNA in 11 cases gastric benign lesion tissues.There was significant difference(P<0.01)among the three groups of patients.The amount of Tiaml mRNA in gastric cancer tissues was much higher than in the para-cancerous tissues.There was no obvious correlation between the expression level and sex,age of patients,as well as the sizes or primary location of gastric cancer(P>0.05),on the other hand,the expression quantity of Tiaml mRNA in gastric cancer was significantly correlated with metastasis into lymph node,the extent of invasion,differentiated degree of histologic type,and the stages of TNM(P<0.05). Conclusions FQ-RT-PCR is a sensitive and specific assay for quantificating expression of Tiam1.There exists the correlation between Tiam1 expression and gastric cancer biological behaviours.Tiam1 may become a novel target for gastric cancer’s gene therapy.更多还原