【摘要】 目的应用结核分枝杆菌直接检测（MTD）试剂盒对临床痰液样本及分离的结核分枝杆菌复合群,进行检出率和常规菌型鉴定法符合率的比较和评价。方法将10株标准菌种(其中1株为 H₃₇Rv、9株为非结核分枝杆菌)、94株临床分离菌株（其中48株经常规生化方法分型鉴定为结核分枝杆菌,46株分型法鉴定为非结核分枝杆菌）和40份临床痰标本分别用法国生物梅里埃公司生产的 AMPLIFIED-MTD 试剂盒进行检测。结果结核分枝杆菌复合群检测均呈阳性,非结核分枝杆菌检测均呈阴性,符合率均达到100%。临床痰标本用 MTD 检测的阳性检出率为65%（26/40）,明显高于直接涂片法的12%（5/40）、培养方法的12%（5/40）、集菌涂片法的25%（10/40）。结论MTD试验是一套新的生物学检测技术,具有快速、灵敏的特点。更多还原

【Abstract】 Objective To evaluate the capacity of MTD method to distinguish between Myeobacterium tuberculosis and nontuberculosis mycobacteria.Methods Ten standard strains(including 1 H₃₇Rv strain and 9 nontuberculosis mycobacteria strains),94 clinical strains（including 48 Mycobacterium tuberculosis and 46 nontuberculosis mycobaeteria strains）and 40 sputum specimens were tested by MTD method（AMPLIFIED-MTI）)and traditional methods.The results of these methods were compared.Results For all Myeobacterium tuberculosis and nontuberculosis mycobacteria strains,the agreement of MTD method and traditional method was 100%.And the positive detectable rate for sputum samples was 65% that was obviously higher than that for the direct smear（5/40）,concentration smear（10/ 40）or culture（5/40）.Conclusion MTD is a rapid test for identification of Mycobacterium tuberculosis and nontuberculosis mycobaeteria with high sensitivity and specificity.更多还原