【摘要】 目的探讨头皮屑相关疾病中头皮屑白介素1α(IL-1α)、白介素1β(IL-1β)、白介素1受体拮抗剂(IL-1ra)和白介素8(IL-8)表达水平及其意义。方法Sebutape胶带粘贴收集15例头皮糠疹、22例脂溢性皮炎、15例头皮银屑病、15例正常人对照者的头皮屑,经超声制备头皮屑萃取液,应用ELISA方法测定其中的细胞因子,BCA增强法检测其总蛋白(TP)含量。结果IL-1α/TP在头皮糠疹组高于正常人对照组(P<0.05),脂溢性皮炎组显著高于正常人对照组(P<0.01)。IL-1β/TP在各组间差异均无统计学意义(P>0.05)。头皮银屑病组头皮屑中的IL-1ra/TP及IL-1ra/IL-1α比值较正常人对照组显著升高(P<0.001)。IL-8/TP在脂溢性皮炎组、头皮银屑病组中含量均显著高于正常人对照组(P<0.001)。结论在头皮糠疹、脂溢性皮炎和头皮银屑病中,头皮屑的产生可能与IL-1α及/或IL-8介导的炎症反应有关。更多还原

【Abstract】 Objective To investigate the difference in the content of interleukin-1α(IL-1α), interleukin-1β(IL-1β),interleukin-1 receptor antagonist (IL-1ra),and interleukin-8 (IL-8) in scalp scales,and its significance in scalp scales-related diseases.Methods Scale samples were collected from patients with pityriasis capitis (n=15),seborrheic dermatitis (n=22),scalp psoriasis (n=15),and healthy control subjects (n=15) by Sebutape tape adhesion,then ffeezed at -80℃.One day later,the samples were subjected to ultrasonic degradation in D-phosphate buffered saline,and freezed again at -80℃. Three days later,the samples were thawing,then ELISA was used to measure the concentration of IL-1α, IL-1β,IL-1ra,and IL-8 in the extract fluid,and BCA method to measure the level of total protein (TP).To account for differences in protein loading on the tapes,all cytokine results were normalized using the TP amounts recovered in tape extracts.Results The IL-1α/TP level in the dandruff of patients with pityriasis capitis and those with seborrheic dermatitis were significantly higher than that in the normal scalp scales (P<0.05,P<0.01,respectively).There was no significant difference in IL-1β/TP level among all the groups (P>0.05).Both the IL-1ra/TP level and IL-1ra/IL-1αratio in the dandruff of scalp psoriasis patients were significantly higher than those in the normal controls (both P<0.001).The IL-8/TP level was significantly increased in the dandruff of patients with seborrheic dermatitis and those with scalp psoria- sis in comparison with normal scalp scales (P<0.01,P<0.001,respectively).Conclusion These results suggest that IL-1α-and/or IL-8-mediated inflammatory responses may contribute to the pathogenesis of dan- druff in pityriasis capitis,seborrheic dermatitis,and scalp psoriasis.更多还原