【摘要】 目的:研究江苏地产白首乌C21甾苷(CSG)对D-半乳糖(D-gal)衰老模型小鼠的影响。方法:小鼠分为正常组,模型组,CSG低、中、高剂量组(10,20,40 mg.kg-1.d-1)和维生素E(Vit E)对照组(100 mg.kg-1.d-1),除正常组外均采用D-gal 10 mg.kg-1颈背部皮下注射,试验期为56 d。检测小鼠血清、心、肝、脑组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及端粒酶活性。结果:与正常组相比,模型组小鼠血清、心、肝、脑组织SOD活性降低,含量增加,血清、心、肝端粒酶活性降低(P<0.01);与模型组比较,3个CSG组及Vit E组小鼠血清、心、肝、脑SOD活性升高,MDA含量减少(P<0.01);低剂量CSG组及Vit E组小鼠血清端粒酶活性有升高的趋势,但差异不显著,中、高剂量CSG组小鼠血清端粒酶活性升高(P<0.01);3个CSG组及Vit E组均能升高小鼠心脏端粒酶活性(P<0.01);3个CSG组及Vit E组小鼠肝及脑组织端粒酶活性无明显变化。结论:CSG能拮抗自由基损伤,提高D-gal衰老模型小鼠血清、心、肝、脑组织SOD活性,减少MDA含量,提高血清、心组织端粒酶活性。更多还原

【Abstract】 Objective:To study the effect of C21 steroidal glycoside(CSG) from the root of Cynanchum auriculatum from Jiangsu on D-galactose(D-gal) induced aging model mice.Method: D-gal aging mouse model was established by cervicodorsal region subcutaneous injection with D-gal once a day for eight successive weeks.The mice in the normal control group(NCG,non-modeled) and the model control group(MCG,modeled but untreated) were treated with 1% CMC-Na.The model mice in the low,middle and high-dose CSG and Vitamin E treated groups were treated with a dose of(10,20,40,100 mg·kg-1) per day,respectively.The SOD activity,MDA content and telomerase activity in serum,heart,liver and brain tissues of mice were measured.Result: CSG could obviously increase the SOD activity and decrease the MDA level in serum,heart,liver and brain tissues in D-gal aging mice(P<0.01).There was no significant difference between three CSG treated groups and Vitamin E treated groups.In comparison of telomerase activity between MCG and the treated groups,it was shown that there was a significant increase in serum in middle and high dose group,and in heart tissues in CSG and Vit E treated groups,but was not in liver and brain tissue.Conclusion: This study demonstrates that CSG can antagonize free radical injury,increase the SOD activity and decrease the MDA content of serum,heart,liver and brain in D-gal aging mice,and increase the telomerase activity in serum and heart tissues but not in liver and brain tissue.更多还原