【摘要】 目的:探讨西兰花中异硫氰酸盐(isothiocyanates,ITCS)诱导人肝癌细胞HepG-2凋亡过程中的作用及其可能机制。方法:不同浓度ITCS处理人肝癌细胞HepG-2,应用MTT法检测ITCS对HepG-2细胞增殖的影响;流式细胞仪检测活性氧、线粒体膜电位(Δψm)和细胞亚二倍体凋亡峰变化情况。结果:ITCS可明显抑制HepG-2细胞增殖;15,30,60,120,240μg.mL-1的ITCS作用于HepG-2细胞24 h后,细胞内活性氧分别为(23.1±1.8)%,(53.3±3.3)%,(57.9±2.0)%,(79.9±3.4)%,(93.4±2.6)%;Δψm分别为(94.8±5.5)%,(91.8±5.4)%,(66.0±5.6)%,(65.5±6.6)%,(44.3±2.7)%;60,120,240μg.mL-1的ITCS作用于HepG-2细胞48 h后可见细胞凋亡率分别为(16.6±2.8)%,(21.9±4.4)%,(70.2±5.3)%。结论:ITCS能够通过刺激HepG-2细胞内活性氧的产生,损伤线粒体,使其膜电位下降,引起HepG-2细胞凋亡。更多还原

【Abstract】 Objective: To investigate the apoptosis effect of isothiocyanates(ITCS) on human liver cancer cells HepG-2,and its mechanism.Method: HepG-2 cells were treated with different concentrations of ITCS.MTT assay was used to evaluate the influence of ITCS on cell proliferation.Flow cytometry was used to test ROS levels,intracellular mitochondrial transmembrane potential(Δψm),and hypodiploid apoptosis peak in HepG-2 cells.Result: ITCS obviously inhibited proliferation of HepG-2 cells.When treated with 15,30,60,120,240 μg·mL-1 of ITCS for 24 h,ROS levels were(23.1±1.8)%,(53.3±3.3)%,(57.9±2.0)%,(79.9±3.4)%,(93.4±2.6)% respectively;and Δψm were(94.8±5.5)%,(91.8±5.4)%,(66.0±5.6)%,(65.5±6.6)% and(44.3±2.7)% respectively;when treated with 60,120,240 μg·mL-1 of ITCS for 48 h,cell apoptotic rates were(16.6±2.8)%,(21.9±4.4)% and(70.2±5.3)% respectively.Conclusion: ITCS generates ROS in gastric cancer HepG-2 cells,which causes mitochondrial membrane permeabilization and Δψm decrease,therefore, leads to apoptosis of HepG-2 cells.更多还原