【摘要】 目的观察降钙素对卵巢切除大鼠股骨骨折愈合的作用,为临床上治疗骨质疏松性骨折提供实验依据。方法50只雌性、14周龄SD大鼠共分成5组,每组10只。分成假手术组(Sham,G1),双侧卵巢切除术组(OVX,G2),假手术+骨折组(Sham+F,G3),卵巢切除术+骨折组(OVX+F,G4),卵巢切除+骨折+降钙素药物组(OVX+F+CT,G5),骨折组大鼠均采用右股骨中段横行骨折,髓内针固定;降钙素采用皮下给药,隔日1次(16IU.kg-1)。所有大鼠于术后4周杀死,取右侧股骨标本。然后,分别进行CR摄片、组织形态学观察,并应用双能X线骨密度仪(DEXA)测量右股骨整体、远段和中段骨密度以及BMP-2免疫组化观察,并应用病理图像分析仪对BMP-2免疫组化进行光密度测量。结果(1)OVX组与Sham组比较,BMD显著下降。(2)OVX+F+CT组与OVX+F组比较:骨痂mBMD显著增高;BMP-2的表达无显著性差异。结论降钙素对OVX大鼠股骨骨折具有明显促进骨折愈合的作用,加速编织骨向板层骨的演变过程。更多还原

【Abstract】 Objective To observe and evaluate the effects of Calcitonin on fracture healing process and to provide supportive evidences of experiment for the treatment of osteroporosic fracture clinically. Methods Fifty 14-week-old female Sprague-Dawley rats were divided randomly into five groups of ten rats of each. Five types treatment had been operated to the five allocated groups respectively sham-operated group(Sham),bilateral ovariectomized group(OVX),sham-operated and femur fracture group(Sham+F),bilateral ovariectomized and femur fracture group(OVX+F),bilateral ovariectomized and femur fracture and administration of calcitonin group(OVX+F+CT). In groups which fractures needed right femoral diaphysis was fractured transversely at the midshaft and fixed with intramedullary nail. The rats in OVX+F+CT group were treated with calcitonin by subcutaneous injections (16 IU·kg~ -1 ) once per two days for 4 weeks. All the rats were killed in 4 weeks after operation. Right femur were removed for the observation of radiology and histology for the measurement of general/mid section/end section bone mineral density (BMD) and immunohistochemistry assessment by dual-energy X-ray absorptiometry(DEXA) and by light symptom visual analysis. Results (1) The mean tBMD of the OVX group was significantly lower than that of the Sham group.(2)The mean mBMD of the OVX+F+CT group was significantly higher than that of the OVX+F group; The woven bones have remodeled into lamellar bones and cartilage islands was rarely observed. No significant diffierence in expression of BMP-2 between the OVX+F and OVX+F+CT groups in callus were noted. Conclusion Calcitonin obviously accelerates the fracture healing in ovariectomied rats and improves woven bones to remodel into lamellar bones.更多还原